How much should I load HyperLadder?
Vortex the tube and use 5 L per lane. There is no need to add loading buffer to the ladder. Use the 5x loading buffer for sample loading, under no circumstances should it be used for diluting the ladder. The blue dye in the loading buffer runs at approximately 4 bp on a 1% gel.
What does KB mean in DNA Ladder?
1 kb plus DNA ladder is a DNA size standard consisting of DNA fragments of length ranging from 0.5 kb to 10 kb used for the determination of the size of double-stranded DNA fragments of length ranging from 250 bp to 25,000 bp. 1 kb plus DNA ladder consists of 14 individual chromatography-purified DNA fragments.
What is the purpose of the HyperLadder?
HyperLadder Range Bioline offers a wide range of molecular weight markers with loading buffer in a single tube, enabling accurate DNA sizing and mass determination of DNA. Each ladder includes one, two or three higher-intensity reference bands for easy identification and orientation.
What is a bioline HyperLadder?
HyperLadder™ 100bp is a molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% agarose gels.
What is KB in electrophoresis?
A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb).
How do you make a 1kb DNA ladder?
If you want to prepare a working stock of the 1 kb ladder with loading dye, measure amount of ladder in the tube and add the loading dye accordingly. Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).
What is the function of a ladder in gel?
A DNA ladder is a solution of DNA molecules of different lengths used in agarose or acrylamide gel electrophoresis. It is applied as a reference to estimate the size of unknown DNA molecules that were separated based on their mobility in an electrical field through the gel.
What is the function of the ladder in gel electrophoresis Labster?
A nucleic acid “ladder” or molecular weight standard sample is a mix of DNA or RNA fragments with known lengths. It is used as a scale for determining the lengths of unknown nucleic acid fragments when performing gel electrophoresis experiments.