What is an oligo primer?
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Researchers use oligos that are anywhere between 20-35 bases long called primers to start copying or amplifying. This DNA primer is usually custom designed to match the target sequence of DNA for copying.
What is oligonucleotide pool?
Oligo pools, a diverse collection of oligonucleotides, allow the precise design and synthesis of thousands of user-defined sequences in parallel that can be utilized in high-throughput screening experiments for identification of novel gene mutations, optimization of protein structure and function, or for drug discovery …
How much do oligos cost?
Single-stranded DNA
| Product | Length | Pricing |
|---|---|---|
| 25 nmole DNA Plate Oligo | 15 – 60 Bases | $0.20 USD / Base |
| 100 nmole DNA Plate Oligo | 10 – 90 Bases | $0.33 USD / Base |
| 250 nmole DNA Plate Oligo | 5 – 100 Bases | $0.56 USD / Base |
| 1 umole DNA Plate Oligo | 5 – 100 Bases | $1.11 USD / Base |
What is Mer in oligo?
The length of the oligonucleotide is usually denoted by “-mer” (from Greek meros, “part”). For example, an oligonucleotide of six nucleotides (nt) is a hexamer, while one of 25 nt would usually be called a “25-mer”.
What is the difference between oligo and primer?
The key difference between random primers and oligo dT is that the random primer is a mixture of all possible hexamer oligonucleotide sequences, while the oligo dT primer consists of a single-stranded stretch of 12–18 deoxythymidines.
What is oligo yield?
The yield corresponds to the amount of final product recovered at the end of the synthesis and purification processes.
What is an Ultramer oligo?
Ultramer DNA Oligos are long, single- and double-stranded synthetic DNA sequences. They are manufactured with an optimized synthesis platform that achieves consistent coupling efficiencies over 99.5%.
What is oligo manufacturing?
In its simplest description, oligonucleotide manufacturing consists of only a few, basic unit operations: solid-phase synthesis, purification, and isolation. The earliest syntheses of oligonucleotide drug substance were performed via solid-phase couplings on controlled pore glass (CPG) in sparged bed reactors.
Why are primers called oligonucleotides?
The term oligonucleotide is derived from the Greek “oligo,” which means few or small. The length of the oligonucleotide is usually denoted by the term “mer,”which is Greek for “part.” Oligonucleotides are used as probes for detecting specific sequences that are complementary to the oligonucleotides.