What is generalized transduction in microbiology?
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Generalized transduction is the process by which any bacterial gene may be transferred to another bacterium via a bacteriophage, and typically carries only bacterial DNA and no viral DNA. In essence, this is the packaging of bacterial DNA into a viral envelope.
What is Generalised transduction?
Generalized transduction occurs when random pieces of bacterial DNA are packaged into a phage. It happens when a phage is in the lytic stage, at the moment that the viral DNA is packaged into phage heads. If the virus replicates using ‘headful packaging’, it attempts to fill the head with genetic material.
What are the steps in generalized transduction?
Generalized transduction: Mechanism
- Attachment/adsorption of bacteriophage to the bacteria.
- Penetration of phage DNA.
- Replication of phage DNA/RNA.
- Synthesis of nucleic acid and proteins.
- Assembly of phage protein and nucleic acid.
- Release of mature bacteriophage.
What are the difference between generalized and transduction?
The key difference between generalized and specialized transduction is that generalized transduction is done by virulent bacteriophages in which bacterial cell is lysed when new bacteriophages are released while specialized transduction is done by temperate bacteriophages in which bacterial cell is not lysed, and viral …
What is the difference between generalized transduction and transformation?
Key points: In transformation, a bacterium takes up a piece of DNA floating in its environment. In transduction, DNA is accidentally moved from one bacterium to another by a virus.
Does transduction require recA?
Generalized Transduction If the bacterial DNA in the phage is from the bacterial chromosome, the DNA recombines with the homologous DNA of the bacterial recipient to generate stable transductants. This process requires a host recombinase, recA.
What is the relationship between generalized and specialized transduction and the lytic and lysogenic cycles?
Generalized transduction occurs when a random piece of bacterial chromosomal DNA is transferred by the phage during the lytic cycle. Specialized transduction occurs at the end of the lysogenic cycle, when the prophage is excised and the bacteriophage enters the lytic cycle.
What is the result of generalized transduction?
Generalized Transduction During this stage, random pieces of bacterial DNA are mistakenly packaged into a phage head, resulting in the production of a transducing particle.
How is generalized transduction different from specialized transduction quizlet?
How is generalized transduction different from specialized transduction? Generalized transduction is initiated during lytic cycle of a virulent bacteriophage; specialized transduction is initiated during the lysogenic cycle of a temperate bacteriophage.
What is the difference between generalized and specialized transduction?