What is the Assay buffer in ELISA?

What is the Assay buffer in ELISA?

ELISA Assay Buffer (5X) is intended for use as: blocking reagent for ELISA plates, diluent for ELISA standards and samples, diluent for the detection antibody, and diluent for the HRP conjugate. This buffer is recommended when using Invitrogen Antibody Pairs or when developing a sandwich ELISA.

Why is sodium azide used in FACS buffer?

Flow Cytometry Staining Buffer (FACS Buffer) The buffer contains sodium azide as preservative and animal serum proteins (FBS/BSA) to help minimizing non-specific binding of antibodies.

How do I create a FACS buffer?

5 Ingredients to Consider in FACS Buffer

1. 2- Supplement with FCS (1-10%) or BSA (0.1-1%) Serum proteins protect cells from apoptosis, prevent non-specific staining and also prevent cells from sticking to the FACS tubes.
2. 3- Include EDTA (0.5-5 mM)
3. 4- Slip in some DNase I (25-50µg/ml)
4. 5- Add sodium azide (0.1-1%)

What is in cell staining buffer?

Cell Staining Buffer contains bovine calf serum as a protein carrier to reduce non-specific binding of antibodies and fluorochrome reagents to target cells. It also contains a metabolic inhibitor, sodium azide (NaN3), to inhibit patching and capping of cell surface antigens.

What is assay buffer?

Assay Buffer is a buffered protein and detergent solution intended for use in dissociation- enhanced time-resolved fluoroimmunoassays (DELFIA) that include Eu/Sm/Tb-labeled antibodies or antigens. It is optimized to give a minimum non-specific background in solid phase assays.

What is an assay buffer used for?

Assay buffers are mixtures of buffers, salts, detergents, protein, preservatives, and coloring agents. These buffers are used in ELISA assays for dilution of standards, samples, and reagents, and for blocking of non-specific interactions.

How much sodium azide is in a FACS buffer?

Facs buffer is usually used to stain extracellularly but it is generally speaking the buffer that you use to resuspend your cells before and after the staining. Usually Facs Buffer is PBS 1%BSA (or 4%FCS) 0,05% Sodium Azide.

What are the components of FACS buffer?

Usually Facs Buffer is PBS 1%BSA (or 4%FCS) 0,05% Sodium Azide.

How do you save a FACS buffer?

Wash the cells 3 times by centrifugation at 1500 rpm for 5 minutes and resuspend them in 200 μl to 1ml of ice cold FACS buffer*. Keep the cells in the dark on ice or at 4°C in a fridge until your scheduled time for analysis.

How do you stain a flow cytometry?

Dilute the appropriate fluorophore-labeled secondary detection reagent in 100 µL of Flow Cytometry Staining Buffer and add to cells. Incubate for at least 30 minutes at 2–8°C or on ice. Protect from light. Wash the cells by adding Flow Cytometry Staining Buffer.

Is assay buffer hazardous?

May be harmful by inhalation, ingestion, or skin absorption. May cause eye, skin, or respiratory system irritation.