Where do we observe polytene chromosome in Chironomus larvae?
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History. Polytene chromosomes were originally observed in the larval salivary glands of Chironomus midges by Édouard-Gérard Balbiani in 1881. Balbiani described the chromosomal puffs among the tangled thread inside the nucleus, and named it “permanent spireme”.
Why are polytene chromosomes in fly larvae?
Polytene chromosomes are usually found at the interphase nuclei of some tissue of the larvae of flies. Polytene chromosomes are considered to be very useful for the analysis of many facets of eukaryotic interphase chromosome organization and the genome as a whole.
Where would you expect the polytene chromosomes to be located in the blowfly larvae?
Figure 1 shows them to be positioned between the fourth and ninth visible segment of the larva on the ventral side.
What is polytene chromosome write the procedure of squash preparation of Drosophila larvae for polytene chromosome?
Polytene chromosome squash preparation: Rinse larvae with water and transfer to PBS in a tissue culture dish for dissection. Grasp the tip of the mouth hooks with one pair of forceps, hold the body about 2/3 of the way down with the other pair, and pull on the mouth hooks so the salivary glands are exposed.
Which organ is generally used to find the polytene chromosome in Drosophila?
Further, the polytene chromosome located within the larval salivary glands of Drosophila, provide scientists the unique ability to directly visualize structural changes that result from the epigenetic modifications of chromatin [10,11].
Who discovered chromosomes in salivary gland of Chironomus larva?
Balbiani discovered special type of chromosome from the salivary gland of Chironornus larva, which are recognized by the presence of.
What does polytene mean?
Definition of polytene : relating to, being, or having chromosomes each of which consists of many strands with the corresponding chromomeres in contact.
What is meant by polytene chromosome?
a giant, cross-banded chromosome that results from multiple replication of its genetic material with the duplicated chromatin strands remaining closely associated.
What is polytene chromosome and how they are formed?
Polytene chromosomes develop from the chromosomes of diploid nuclei by successive duplication of each chromosomal element (chromatid) without their segregation. The newly formed chromatids remain associated lengthwise and together form a cable-like structure, referred to as polytene chromosomes.